Figure 9

Knocking-down duDDX1 expression reduces induction of some major innate immune and anti-viral gene expression after NDRV infection. DEF cells were transfected with Si-duDDX1-1 as the experimental group, and DEF cells were transfected with Si-NC as the control group. The cells were stimulated with 1 TCID₅₀/mL NDRV after 36 hpt. Cells were collected and analyzed for inducible gene expression at 24, 36, and 48 hpi. The relative expression of gene mRNA were calculated using the 2^−ΔΔCT method with GAPDH serving as a normalization gene and mean control values as baseline reference. Data are represented as the mean value ± SE of three experiments. A student t test was performed to assess the difference. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.