Figure  6

Eno activates p38/ERK MAPK-eIF4E signalling to increase HSPD1 expression by binding to RPSA. A Eno treatment increased expression of RPSA and HSPD1 in PBMECs; B Knockdown of RPSA decreased Eno-promoted HSPD1 expression, but knockdown of HSPD1 did not decrease Eno-promoted RPSA expression; C Western blotting analysis of p38, ERK, and eIF4E of Eno-treated cells; D Analysis of MAPK activation and its effect on HSPD1 expression in Eno-treated cells; E Analysis of HSPD1 expression in Eno-treated cells after inhibition of eIF4E; F Analysis of eIF4E activation in Eno-treated cells after inhibition of MAPK signalling; G The effect of RPSA knockout or overexpression on Eno-induced MAPK-signalling activation in cells. RPSA-/-(R): Complementary RPSA; RPSA (Over): RPSA overexpression. (*p < 0.05, **p < 0.01, ^NSp > 0.05).