Figure  3

Analysis of Eno-induced cell apoptosis. A Eno-induced cytotoxicity for PBMECs showed a time-dependent enhancement; B Eno-induced apoptosis for PBMECs showed time- and dose-dependent enhancement by flow cytometry; C, D Eno-induced cleaved caspase-3 expression showed time- and dose-dependent enhancement by Western blotting; E Eno-induced apoptosis is dependent on caspase-8 and caspase-3 activation; Z-DEVD-FMK is a caspase-3 inhibitor; Z-ITED-FMK is a caspase-8 inhibitor; Z-LEHD-FMK is a caspase-9 inhibitor; a, Z-VAD-FMQ is an inhibitor for all caspases; F Blocking Eno of SS2 by Rabbit anti-Eno IgG can inhibit SS2-induced apoptosis. The role of Eno in SS2-induced PBMEC apoptosis; G The role of apoptosis in SS2 breaking into the BBB model; H Immunohistochemical analysis of mouse brains showed that injection with rabbit anti-Eno inhibited cleaved caspase-3 expression caused by SS2 infection (scale bar, 100 μm). (^*p < 0.05, ^**p < 0.01, ^NSp > 0.05).