Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species

Table 2 Primers used for the quantitative real-time PCR.

RNA target	Primers sequence	Accession NO	Amplification efficiency (%)^a	Correlation coefficients (r²)
GAPDH	Forward: 5′-GGTGGTGCTAAGCGTGTTAT-3′	K01458	100.74	0.9917
GAPDH	Reverse: 5′-ACCTCTGTCATCTCTCCACA-3′	K01458	100.74	0.9917
IL-2	Forward: 5′-TAACTGGGACACTGCCATGA-3′	AF000631	102.44	0.9921
IL-2	Reverse: 5′-GATGATAGAGATGCTCCATAAGCTG-3′	AF000631	102.44	0.9921
IL-4	Forward: 5′-ACCCAGGGCATCCAGAAG-3′	AJ621735	99.09	0.9936
IL-4	Reverse: 5′-CAGTGCCGGCAAGAAGTT-3′	AJ621735	99.09	0.9936
IL-10	Forward: 5′-GGAGCTGAGGGTGAAGTTTGA-3′	AJ621614	99.19	0.9923
IL-10	Reverse: 5′-GAAGCGCAGCATCTCTGACA-3′	AJ621614	99.19	0.9923
IL-17	Forward: 5′-ACCTTCCCATGTGCAGAAAT-3′	EF570583	100.24	0.9940
IL-17	Reverse: 5′-GAGAACTGCCTTGCCTAACA-3′	EF570583	100.24	0.9940
IFN-γ	Forward: 5′-AGCTGACGGTGGACCTATTATT-3′	Y07922	103.07	0.9868
IFN-γ	Reverse: 5′-GGCTTTGCGCTGGATTC-3′	Y07922	103.07	0.9868
TGF-β	Forward: 5′-CGGGACGGATGAGAAGAAC-3′	M31160	102.79	0.9815
TGF-β	Reverse: 5′-CGGCCCACGTAGTAAATGAT-3′	M31160	102.79	0.9815
TNF SF15	Forward: 5′-GCTTGGCCTTTACCAAGAAC-3′	NM_001024578	100.57	0.9930
TNF SF15	Reverse: 5′-GGAAAGTGACCTGAGCATAGA-3′	NM_001024578	100.57	0.9930

ISSN: 1297-9716