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Table 2 Primers used for the quantitative real-time PCR.

From: Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species

RNA target Primers sequence Accession NO Amplification efficiency (%)a Correlation coefficients (r2)
GAPDH Forward: 5′-GGTGGTGCTAAGCGTGTTAT-3′ K01458 100.74 0.9917
Reverse: 5′-ACCTCTGTCATCTCTCCACA-3′
IL-2 Forward: 5′-TAACTGGGACACTGCCATGA-3′ AF000631 102.44 0.9921
Reverse: 5′-GATGATAGAGATGCTCCATAAGCTG-3′
IL-4 Forward: 5′-ACCCAGGGCATCCAGAAG-3′ AJ621735 99.09 0.9936
Reverse: 5′-CAGTGCCGGCAAGAAGTT-3′
IL-10 Forward: 5′-GGAGCTGAGGGTGAAGTTTGA-3′ AJ621614 99.19 0.9923
Reverse: 5′-GAAGCGCAGCATCTCTGACA-3′
IL-17 Forward: 5′-ACCTTCCCATGTGCAGAAAT-3′ EF570583 100.24 0.9940
Reverse: 5′-GAGAACTGCCTTGCCTAACA-3′
IFN-γ Forward: 5′-AGCTGACGGTGGACCTATTATT-3′ Y07922 103.07 0.9868
Reverse: 5′-GGCTTTGCGCTGGATTC-3′
TGF-β Forward: 5′-CGGGACGGATGAGAAGAAC-3′ M31160 102.79 0.9815
Reverse: 5′-CGGCCCACGTAGTAAATGAT-3′
TNF SF15 Forward: 5′-GCTTGGCCTTTACCAAGAAC-3′ NM_001024578 100.57 0.9930
Reverse: 5′-GGAAAGTGACCTGAGCATAGA-3′
  1. a Amplification efficiency (%) = (10−1/slope − 1) × 100%.