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Figure 3 | Veterinary Research

Figure 3

From: Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species

Figure 3

Transcription and expression detection of recombinant plasmid pVAX1-EmROM2 at the injection site. A Transcription detection of pVAX1-EmROM2 by RT-PCR; lane M: DL 2000 DNA marker; lane 1: product of EmROM2 from pVAX1-EmROM2-injected muscle; lane 2: pVAX1-injected muscle control; lane 3: non-injected muscle control; B Expression detection of pVAX1-EmROM2 by Western blot; lane M: standard protein molecular weight marker; lane 1: pVAX1-EmROM2-injected muscle recognized by rat anti-rEmROM2 serum; lane 2: pVAX1-EmROM2-injected muscle recognized by negative rat serum.

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