Figure 3

Expression levels of molecules in the ChTLR15/ChNF-κB-ChNLRP3/ChIL-1β signaling pathway in DF1 cells stimulated by E. tenella sporozoites. A Monolayer DF1 cells were stimulated by purified E. tenella sporozoites. At 0, 2, 4, 6, and 8 h post-stimulation, the mRNA levels of ChTLR15, ChMyD88, ChNF-κB, ChNLRP3, ChCaspase-1, ChIL-1β and ChIL-18 in DF1 cells were quantified by quantitative real-time PCR (qPCR). Chicken β-actin was used as a reference gene. B Protein levels of ChTLR15 and ChNLRP3 in DF1 cells were detected by Western blot at 0, 2, 4, 6, and 8 h post-stimulation. C Protein levels of ChIL-1β in culture supernatant were analyzed using an ELISA kit (Jiancheng Bioengineering Institute, Nanjing, China) at 0, 2, 4, 6, and 8 h post-stimulation. Highly significant differences (p < 0.01) between numbers with different capital letters. Significant differences (p < 0.05) between numbers with different lowercase letters. No significant difference (p > 0.05) between numbers with the same letter.