Skip to main content
Figure 5 | Veterinary Research

Figure 5

From: Systemic and intestinal porcine epidemic diarrhea virus-specific antibody response and distribution of antibody-secreting cells in experimentally infected conventional pigs

Figure 5

S/P ratios of PEDV IgG antibody in culture supernatants of MNCs isolated from the blood, spleen and MLN of PEDV-inoculated pigs, with or without in vitro stimulation. MNCs were isolated from the blood (A), spleen (B) and MLN (C) of PEDV inoculated pigs. MNCs were cultured for 4 days to evaluate the responses of specific ASCs. In the other wells, the MNCs were cultured with R848 for 4 days for the stimulation of memory B cells. Cell supernatants were collected, and PEDV-specific antibodies in the supernatants were detected by an IgG ELISA. *p < 0.05 and **p < 0.01 vs. the unstimulated group (significant and highly significant differences, respectively) by Dunnett's test. *p < 0.05 for unstimulated vs. stimulated groups, by one-tailed Student's t-test. Black bar: without in vitro stimulation. Open bar: with in vitro stimulation.

Back to article page

Veterinary Research

ISSN: 1297-9716