Figure 2From: N-linked glycosylation at site 158 of the HA protein of H5N6 highly pathogenic avian influenza virus is important for viral biological properties and host immune responsesViral distribution and cytokine production in respiratory tract of mice. A Six-week-old SPF BALB/c mice (three/group) were inoculated intranasally with 106 EID50 of RY6 and RY6-160T, and respiratory tract tissues were collected at 3, 6, 9, 12, 20, 24, 72 hpi for virus titration with RT-qPCR. Relative mRNA levels of HMGB1, IL10, and TNFα in the infected mouse turbinates B and lungs C. All values were normalized to Gapdh and are expressed as fold change compared with controls. Values represent means ± SD from three independent experiments. (*p < 0.05, **p < 0.01, ***p < 0.001).Back to article page