Figure 3From: Nanobody Nb6 fused with porcine IgG Fc as the delivering tag to inhibit porcine reproductive and respiratory syndrome virus replication in porcine alveolar macrophagesCellular uptake of Nbs-pFc by PAM. A IFA, B Western blot, and C flow cytometry analyses of Nbs-pFc entry into PAM at different concentrations. PAM were treated with 0–20 μM Nbs-pFc for 2 h. D IFA and E Western blot analyses of uptake of 20 μM Nb6-pFc into PAM at different time points of incubation.Back to article page