Apoptosis assay of chicken splenic-derived dendritic cells (chSPDCs) in response rEaGAPDH. A Apoptosis of chSPDCs was measured by flow cytometry after stained with annexin-V and PI. Bar graphs represent percentage of early stage apoptosis (B), late stage apoptosis (C) and total (early + late) apoptotic cells (D). Data are presented as the mean ± SEM (n = 3) from triplicate experiments. “ns” designate treatment groups are non-significantly differ to that of negative control groups.