Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1

He, Tianqiong; Wang, Mingshu; Cheng, Anchun; Yang, Qiao; Jia, Renyong; Wu, Ying; Huang, Juan; Chen, Shun; Zhao, Xin-Xin; Liu, Mafeng; Zhu, Dekang; Zhang, Shaqiu; Ou, Xuming; Mao, Sai; Gao, Qun; Sun, Di; Wen, XinJian; Tian, Bin; Liu, Yunya; Yu, Yanling; Zhang, Ling; Pan, Leichang; Chen, Xiaoyue

doi:10.1186/s13567-020-00859-w

Veterinary Research

Table 1 Sequences and primer pair characteristics

From: Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1

Primer	Plasmid	Primer sequence (5′ → 3′)
P₁	pET-32a-UL47	G/GATCCAGACAGCGCCGTAGGTCA
P₂	pET-32a-UL47	C/TCGAGAACTATTGCCGGATTAACAGG
P₃	DEV UL47 (RT-PCR)	AACGGAGTTGCTTGGAGAACA
P₄	DEV UL47 (RT-PCR)	TGGGCGATGAAACAGAGTAGG
P₅	Duck β-actin (RT-PCR)	CCGGGCATCGCTGACA
P₆	Duck β-actin (RT-PCR)	GGATTCATCATACTCCTGCTTGCT
P₇	DEV UL54 (RT-PCR)	GAACAACCGCCGAACAC
P₈	DEV UL54 (RT-PCR)	TCAAACATCCGCCTCAA
P₉	DEV UL13 (RT-PCR)	GCCACCAACCCTACCAAG
P₁₀	DEV UL13 (RT-PCR)	GTCGTCAGCCCATCACCA
P₁₁	DEV Us2 (RT-PCR)	AGACGGTTCCGAAAGTACAG
P₁₂	DEV Us2 (RT-PCR)	TCGGCAGCACCAATAATCC
P₁₃	pcaggs-UL47-Flag	CATCATTTTGGCAAAG/AATTCGCCACCATGGCCATGGATAAATCACGAAGAC
P₁₄	pcaggs-UL47-Flag	TTGGCAGAGGGAAAAA/GATCTTTACTTGTCATCGTCGTCCTTGTAATCATGTAACTCTCTCCGCCC
P₁₅	pcasggs-STAT1-myc	CATCATTTTGGCAAAG/AATTCGCCACCATGGCCGAGCAGAAACTCATCTCTGAAGAGGATCTGATGACTCAGTGGTACCAG
P₁₆	pcasggs-STAT1-myc	GGCAGAGGGAAAAA/GATCTTTAAGTTGAATATGCTGAACAC
P₁₇	pEGFP-C2-40-50	AATTCAGGAGAAGTGGTAAGAGACGTACACTTGACAGGG
P₁₈	pEGFP-C2-40-50	GATCCCCTGTCAAGTGTACGTCTCTTACCACTTCTCCTG
P₁₉	pEGFP-C2-768-777	AATTCAAAGCATTAAAACGACGTTTGACTGGTGGGG
P₂₀	pEGFP-C2-768-777	GATCCCCCACCAGTCAAACGTCGTTTTAATGCTTTG
P₂₁	pEGFP-C2-40-50-β-gal	CTCGAGCTCAAGCTTCG/AATTCAGGAGAAGTGGTAAGAGACGTACACTTGACAGGATGAGCGAAAAATACATCG
P₂₂	pEGFP-C2-40-50-β-gal	CAGTTATCTAGATCCGGTG/GATCCTTATTTTTGACACCAGAC
P₂₃	pEGFP-C2-768-777-β-gal	CTCGAGCTCAAGCTTCG/AATTCAAAGCATTAAAACGACGTTTGACTGGTGGGATGAGCGAAAAATACATCG
P₂₄	pEGFP-C2-768-777-β-gal	CAGTTATCTAGATCCGGTG/GATCCTTATTTTTGACACCAGAC
P₂₅	pcDNA3.1 (+)-Flag-UL47Δ40-50-768-777	GCTTGGTACCGAGCTCG/GATCCGCCACCATGGATTACAAGGATGACGACGATAAGATGGATAAATCACGAA
P₂₆	pcDNA3.1 (+)-Flag-UL47Δ40-50-768-777	GTTTAAACGGGCCCTCTAGAC/TCGAGTTAATGTAACTCTCTCCGC
P₂₇	pcDNA3.1-C-UL47-LgBiT	CTGTTGGTAAAGCCACCA/GATCTGCCACCATGGATAAATCACGAAG
P₂₈	pcDNA3.1-C-UL47-LgBiT	CCGCTCCCGCCACCACCGC/TCGAGGGCTTGTCATCGTCGTCCTTGTAATCATGTAACTCTCTCCGCCC
P₂₉	pcDNA3.1-N-STAT1-SmBiT	GAGCGGAGGTGGAGGC/TCGAGCATGACTCAGTGGTACCAG
P₃₀	pcDNA3.1-N-STAT1-SmBiT	CCGCCCCGACTCTAGAA/GATCTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCAGTTGAATATGCTGAACAC

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ISSN: 1297-9716

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