Skip to main content
Figure 7 | Veterinary Research

Figure 7

From: Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1

Figure 7

DEV UL47 inhibits IFN-β signalling induced by poly(I:C). All transfected samples were collected at 36 h posttransfection. A DEV UL47 inhibited the IFN-β-luc luciferase activation induced by poly(I:C) in DEF cells. DEF cells were co-transfected with IFN-β-luc luciferase reporter plasmid, pRL-TK, pcaggs-UL47-Flag or an empty vector, and then stimulated by 50 µg/mL at 12 h posttransfection. The cells were harvested and detected by dual-luciferase assay at 24 h posttransfection. Protein expression was confirmed by western blotting using mouse anti-Flag MAb. All experiments were performed in triplicate (n = 3). The data was analyzed by one-way ANOVA. ***p < 0.001

Back to article page

Veterinary Research

ISSN: 1297-9716