The residues 40–50 and 768–777 of DEV pUL47 could direct heterologous proteins to the nucleus. All transfected samples were collected after 24 h posttransfection. A DEF cells were transfected with pEGFP-C2-SV40, pEGFP-C2-40-50, pEGFP-C2-768-777 and pEGFP-C2, respectively. B The expression of proteins was confirmed by Western blotting using mouse anti-GFP monoclonal antibody and goat anti-mouse IgG HRP-conjugated antibody (1:5000) (magnification: 200×; scale bar: 10 μm). C DEF cells were transfected with pEGFP-C2-SV40-β-gal, pEGFP-C2-40-50-β-gal, pEGFP-C2-768-777-β-gal and pEGFP-C2-β-gal, respectively. D The expression of proteins was confirmed by western blotting using mouse anti-GFP monoclonal antibody.