Figure 5

Interaction with lectins (A, B) and deglycosylation of carp seminal plasma Wap65-2a and Wap65-2b (C, D). N-Glycosidase F (PNGase F) (+N) and O-glycosidase (+O). Staining for positive Datura stramonium agglutinin (DSA; A) and Maackia amurensis agglutinin (MAA; B) affinities of Wap65-2 (I—fetuin, II—carboxypeptidase Y, III—transferrin, and IV—asialofetuin). Fluorescent staining for glycoproteins using Pro-Q Emerald 300 (C) and for proteins using SYPRO Ruby (D). Lane identities: S—molecular mass marker, 1—Wap65-2a (control), 2—Wap65-2a incubated with O-glycosidase, 3—Wap65-2a incubated with N-glycosidase, 4—Wap65-2b (control), 5—Wap65-2b incubated with O-glycosidase, and 6—Wap65-2b incubated with N-glycosidase.