Figure 3
Validation of miRNAs and their mRNA targets expression by quantitative RT-PCR. The expression changes of 10 miRNAs (A) and their mRNA targets (B) in the H9N2 AIV-infected DCs was calculated using the 2−ΔΔCT method and represented as the n-fold change relative to the mock-infected DCs. The 5 s and β-actin genes were used as the reference genes for miRNA and mRNA respectively. C Relationships between miRNAs and mRNA targets. Red indicates the up-regulated miRNAs (circle) and target mRNAs (hexagon); green indicates the down-regulated miRNAs and target mRNAs.