Zinc uptake system ZnuACB is essential for maintaining pathogenic phenotype of F4ac+ enterotoxigenic E. coli (ETEC) under a zinc restricted environment

Table 1 Differential expression of zinc-related genes in the zinc-restricted medium compared to zinc abundant medium

	Log2 fold change	p value	q value	Gene name or gene description
Upregulated genes
Y75_RS09800	1.22902	0.0004	0.001993	znuA, zinc ACB transporter substrate-binding protein
Y75_RS09805	2.28633	0.00005	0.000301	znuCB, Mn²⁺/Zn²⁺ABC transporter ATP-binding protein
Downregulated genes
Y75_RS19925	− 4.1821	0.00005	0.000301	zntA, Zn²⁺/Cd²⁺/Pb²⁺/Hg²⁺ exporting P-type ATPase
Y75_RS02970	− 2.09338	0.00005	0.000301	cusA, cation efflux system protein cusA
Y75_RS02965	− 2.21868	0.00325	0.012234	cusB, cation efflux system protein CusB
Y75_RS02955	− 2.72515	0.0009	0.004022	cusC, cation efflux system protein CusC
Y75_RS17075.	− 1.04709	0.01555	0.044091	zraP, zinc resistance-associated protein, zinc responsive, periplasmic protein with chaperone activity

log2 (fold change): log2(30 μM TPEN-FPKM/1 mM Zn²⁺-FPKM), p-value: p value of difference test, q-value: p value corrected by Benjamini–Hochberg correction method.

ISSN: 1297-9716