Figure 4
Adherence of WT C83902 and C83902 ΔznuACB. A Deletion of C83902 znuACB decreases the adherence to IPEC-J2 cell. An adherence assay of WT C83902 and C83902 ΔznuACB to IPEC-J2 cell after pre-incubation in LB medium. B Zinc restriction led to a significant decrease of adhesion ability of C83902 ΔznuACB, while zinc supplementation can restore its adhesion ability. Bacteria were incubated in 30 μM TPEN pre-treated LB medium and supplemented with 10 μM or 20 μM Zn2+, then adherence assays of WT C83902 and C83902 ΔznuACB to IPEC-J2 cell were performed. The WT strain adhesion index was assumed to be 100%. Data are expressed as mean ± standard deviation of triplicate experiments. Statistically significant differences are indicated as ***p < 0.001 when compared to the WT strain.