Figure 2

Bacterial growth curves. A WT strain and mutants were incubated in LB broth at 37 °C for 10 h with agitation, and OD₆₀₀ optical was measured every 1 h. B WT strain and mutants were incubated in LB broth with 30 μM TPEN at 37 °C for 12 h with agitation. C and D WT strain and mutants were incubated in 30 μM TPEN pre-treated LB broth with 0.5 mM and 2 mM Zn²⁺ at 37 °C for 10 h with agitation, and OD₆₀₀ optical was measured every hour. Data are the means and standard deviations from three independent experiments. *p < 0.05 (Student t test) compared to the corresponding wild type.