Table 1 Primer and probe sequences for qPCR
From: The immunomodulatory effect of cathelicidin-B1 on chicken macrophages
Gene | 5′ → 3′sequence | Accession number |
|---|---|---|
GAPDH | F: GTCAACCATGTAGTTCAGATCGATGA | NM_204305.1 |
R: GCCGTCCTCTCTGGCAAAG | ||
P: AGTGGTGGCCATCAATGATCCC | ||
28S | F: GGCGAAGCCAGAGGAAACT | X59733 |
R: GACGACCGATTTGCACGTC | ||
P: AGGACCGCTACGGACCTCCACCA | ||
IFN-β | F: CCTCCAACACCTCTTCAACACG | KF741874.1 |
R: TGGCGTGTGCGGTCAAT | ||
P: AGCAGCCCACACACTCCAAAACACT | ||
IL-1β | F: GCTCTACTAGTCGTGTGTGATGAG | NM_204524.1 |
R: TGTCGATGTCCCGCATGA | ||
P: CCACACTGCAGCTGGAGGAAGCC | ||
IL-6 | F: GTCGAGTCTCTGTGCTAC | NM_204628.1 |
R: GTCTGGGATGACCACTTC | ||
P: ACGATCCGGCAGATGGTGA | ||
IL-8 | F: GCCCTCCTCCTGGTTTCA | NM_205498.1 |
R: CGCAGCTCATTCCCCATCT | Â | |
P: TGCTCTGTCGCAAGGTAGGACGCTG | Â | |
IL-10 | F: CATGCTGCTGGGCCTGAA | NM_001004414.2 |
R: CGTCTCCTTGATCTGCTTGATG | ||
P: CGACGATGCGGCGCTGTCA | ||
CATH-1 | F: GCTGACCCTGTCCGCGTCA | NM_001001605.3 |
R: GAGGTTGTATCCTGCAATCAC | ||
P: CCTGATGACCAGCGGC | ||
CATH-2 | F: CAAGGAGAATGGGGTCATCAG | NM_001024830.2 |
R: CGTGGCCCCATTTATTCATTCA | ||
CATH-3 | F: CCATGGCTGACCCTGTCC | NM_001311177.1 |
R: TGATGGCTTTGTAGAGGTTGATG | ||
P: CGCAGCCACCGTGTTG | ||
CATH-B1 | F: TGTTCCATAGATCAGCAG | NM_001271172.1 |
R: ATTCAACCACTCCCAGATG | ||
P: TCCACCAGTTGCGGAT |