Figure 4

Cytotoxicity of chicken CATH-B1 and CATH-2. Primary macrophages were incubated with different concentrations (0–10 μM) of CATH-B1 (A) and CATH-2 (B) for 3 h in the presence or absence of 100 ng/mL LPS or 1  ×  10⁶ CFU/mL APEC. Cathelicidins were washed away and cells were maintained in new medium for another 3 h. Metabolic activity was tested using WST-1 reagent. Data are represented as mean  ±  SEM of three independent experiments of triplicate samples per experiment. *P ≤ 0.05; **P ≤ 0.01;***P ≤ 0.005; ****P ≤ 0.001.