Figure 8

rTsP-mediated promotion and anti-rTsP antibody-mediated inhibition of the larval invasion of IECs. Trichinella spiralis ML were first activated into IIL using 5% swine bile for 2 h at 37 °C, after which the IIL were added to an IEC monolayer. The IIL that invaded the IECs were examined under microscopy after culture for 2 h. A Non-invaded IIL coiled on the IEC surface. B Larvae that invaded the IEC monolayer were mobile and migratory. C Non-invaded IIL coiled on the surface of C2C12 cells, which were insensitive to larval invasion and utilized as negative control cells. D Inhibitory effect of anti-rTsP serum on IIL invasion of IECs. E rTsP promoted the IIL invasion of IECs. *P < 0.01 compared to pre-immune serum or BSA. Scale bars: 100 μm.