Table 1 Primer pairs used in this study
Primer name | Sequence in 5′–3′ direction | Location (nt) | Accession number | Product name | Length (bp) |
|---|---|---|---|---|---|
Cap(d41)-XhoI (Forward) | XhoI AGCCTCGAGATGACGTATCCAAGG | 124–702 | AY885225 | Cap(d41) | 579 |
Cap(d41)-BsiWI (Reverse) | PstI TCATCTGCAGTGAGGGTTTAA GTG | ||||
Cap-(d41)-NotI (Forward) | NotI GCGGCCGCTATGAATGGCATC TTCAACACC | 124–702 | AY885225 | Cap(d41) | 579 |
Cap(d41)-SalI (Reverse) | SalI GTCGACTATGGGTTTAAGTGG GGGGTC | ||||
Cap(d41)-XhoI (Forward) | XhoI CTCGAGATGAATGGCATCTTC AACACC | 124–702 | AY885225 | Cap(d41) | 579 |
Cap(d41)-BsiWI (Reverse) | BsiWI CGTACGTATGGGTTTAAGT GGGGGGTC |