Table 1 Primer and probe sequences designed to distinguish between the defective and full viral segments in 95/95(DVG-high) and 95/95(DVG-low) virus isolates
Location | Name of primer/probe | Sequence 5′–3’ |
|---|---|---|
Terminal PB2 | PB2-beg-F | GTCAAATATATTCAATATGGAGAG |
PB2-beg-R | GTGGTCTTTGTTAGTATCTCG | |
PB2-beg-pro | [FAM]-AAGAGATTTGATGTCGCAGTCTC-[TAM] | |
Internal PB2 | PB2-mid-F | AAGAGCAACAGCCATTCTAA |
PB2-mid-R | ATCGCTACTATGATCGCTTC | |
PB2-mid-pro | [FAM]-CAGAAGGCTGATTCAACTGATAG-[TAM] | |
Terminal PA | PA-beg-F | ATGGAAGACTTTGTGCGACAA |
PA-beg-R | AAACAGACTTCTAAGTGTGTGC | |
PA-beg-pro | [FAM]-AATGATTGTCGAGCTTGCGGAAAAG-[TAM] | |
Internal PA | PA-mid-F | AGTGGGCACTTGGTGAGA |
PA-mid-R | ATCCAGCTTGCTAGCGATC | |
PA-mid-pro | [FAM]-TTCATCACTGTCATACTGTCTTAGAT-[TAM] |