Figure 7
Neutralization effect of obtained scFv to CPV. A scFv gene was ligated pcMV-3 vector. B Immunofluorescence analysis of scFv transfected CRFK cells. control: CRFK cells of pcMV-3 without scFv, scFv: CRFK cells of pcMV-3 with scFv, DAPI: staining nuclei. C western blot detection of scFv transfected CRFK cells, control represent empty pcMV-3 transfected CRFK cells. D after transfecting the cells with scFv for 24 h, the cells were infected with CPV (MOI = 0.1). E growth curve of CPV in CRFK cells with scFv and without scFv expression. F The proportion of TCID50 of virus in different cell treatment groups at different time points.