Figure 3

The expression of CD40, CD80 and CD86 on DCs. Wild type (WT) and NLRP3^−/− DCs were treated with MLES (50 μg/mL in vitro for 24 h. Immature DCs were stimulated with sterile phosphate-buffered saline (PBS) as a control. A (I) Representative dot plots for gating on viable cells. (II) The percentages of CD11c⁺ CD40⁺, CD80⁺ and CD86⁺ cells were measured by FACS. B The data represent the means ± standard deviations (SD) of each group (n = 3) of the results from three individual experiments *p < 0.05, **p < 0.01, and ***p < 0.001 as indicated by the line (one-way ANOVA with Tukey’s post-test). These figures are representative of three independent experiments.