Figure 2

Modulation of the ribosomal/TRP53 pathway increases evidence of apoptosis and immune system arrest. The transcription level of four apoptosis genes and two innate immunity genes were measured via RT-PCR following pre-treatment with TRL39 siRNA, RPL9 siRNA, TRP53 inhibitor, or TRP53 activator and infection with Salmonella. Gene expression was normalized to GAPDH transcription level \({2}^{\Delta 40-\mathrm{ct}}\) for A FAS, B RB1, C CASP6, D GADD45A, E NFKB, and F INFG. Error bars represent mean ± SD. * = P < 0.05 (ANOVA test, number of replicates = 3).