Figure 3

Effects of baicalin on the release of HMGB1 into the serum of piglets challenged with G. parasuis. A Determination of HMGB1 production by ELISA. B Determination of the HMGB1 expression level by qPCR. Before G. parasuis challenge, the piglets in the EP group were injected intraperitoneally with EP, and the piglets in the FM group were injected intramuscularly with FM. At 48 h after G. parasuis challenge, blood samples were collected to measure the HMGB1 levels. G. parasuis: HPS. 25–100: the piglets were injected intramuscularly with baicalin at 25, 50, or 100 mg/kg BW. ^##p < 0.01 vs control. *p < 0.05 vs the infection group, and **p < 0.01 vs the infection group.