Figure 2

Effects of baicalin on the release of cytokines into the serum of piglets challenged with G. parasuis. A Determination of cytokine IL1β, IL6, IL8, IL10, and TNF-α production by ELISA. B Determination of cytokine IL1β, IL6, IL8, IL10, and TNF-α expression levels by qPCR. Before G. parasuis challenge, the piglets in the EP group were injected intraperitoneally with EP, and the piglets in the FM group were injected intramuscularly with FM. At 48 h after G. parasuis challenge, blood samples were collected for analysis of the cytokines IL1β, IL6, IL8, IL10, and TNF-α. G. parasuis: HPS. 25–100: piglets were injected intramuscularly with baicalin at 25, 50, or 100 mg/kg BW. ^##p < 0.01 vs control. *p < 0.05 vs the infection group, and **p < 0.01 vs the infection group.