Figure 1

Orally fed CBB-MIX alleviated the colon injury of weaned pigs caused byS. Infantis. A, B Colon histological scores and representative photomicrographs of H&E stained section. C, D Detection of the distribution and expression of occludin (Green) and claudin-1 (Red) in colon tissue by immunofluorescence microscopy. Blue: DAPI. Scale bar, 50 µm. E, Western blot analysis for the expression levels of claudin-1, occludin and ZO-1 proteins in colon tissues from piglets. The lower panel shows the quantitation analysis by Image J software. All these data are presented as mean ± SEM (n = 6), *P < 0.05, **P < 0.01, ***P < 0.001.