MLST-based minimal spanning tree of 88 porcineS. suisisolates. The MLST-based minimum spanning tree is representing the temporal distribution of sequence types determined for S. suis isolates collected during the last 13 years from diseased pigs. The tree was calculated using the goeBURST full MST algorithm in Phyloviz 2.0. Sizes of nodes reflect the number of isolates with a specific MLST profile. Numbers within the nodes indicate the corresponding sequence type. Node colors refer to the year of isolation as represented in the legend. Numbers on lines indicate locus variants between nodes. Black lines indicate single locus variants and grey lines represent multi locus variants. x and y represent isolates with no determined ST due to lacking of housekeeping gene identification.