Table 1 Overview of the experimental set up and results of theCoxiella burnetiiinfection in goats from the start of the experiment till pregnancy confirmation

−7

−1

Arrival in aBSL2; feces sample: C. burnetii DNA neg; serum sample: see Figure 1

Arrival in aBSL2

0

0

Move to aBSL3; inoculation C. burnetii; feces sample: C. burnetii DNA neg; serum sample: see Figure 1

7

1

For all animals EDTA blood sample, feces sample, air sample per room:C. burnetii DNA neg; serum sample: see Figure 1

14

2

For all animals EDTA blood sample, feces sample, air sample per room: C. burnetii DNA neg; serum sample: see Figure 1

21

3

For all animals EDTA blood sample, feces sample, air sample per room: C. burnetii DNA neg; serum sample: see Figure 1

28

4

For all animals EDTA blood sample, feces sample, air sample per room: C. burnetii DNA neg; serum sample: see Figure 1

35

5

For all animals EDTA blood sample, feces sample, air sample per room: C. burnetii DNA neg; serum sample: see Figure 1

42

6

For all animals EDTA blood sample, feces sample, air sample per room: C. burnetii DNA neg; serum sample: see Figure 1

49

7

For all animals EDTA blood sample, feces sample, air sample per room: C. burnetii DNA neg; serum sample: see Figure 1 → move to aBSL2

91

13

11.5 h light

98

14

11 h light

11.5 h light

105

15

10.5 h light

11 h light; intravaginal progesterone sponge administered on D 108

112

16

10 h light; intravaginal progesterone sponge administered on D 115

10.5 h light; 0.2 mL Estrumate i.m. and 1.25 mL Folligonan i.m. on D 117

119

17

10 h light; 0.2 mL Estrumate i.m. and 1.25 mL Folligonan i.m. on D 124

10 h light; progesterone sponge removed on D 119 / breeding on D 120

126

18

Progesterone sponge removed on D 126 / breeding on D 127

133

19

140

20

D 141: air sample per room: C. burnetii DNA neg; for all animals: vaginal swab: C. burnetii DNA neg

147

21

Breeding

154

22

D 155: air sample per room: C. burnetii DNA neg; for all animals: vaginal swab: C. burnetii DNA neg

161

23

Pregnancy check echoscopy

Pregnancy check by echoscopy