Figure 4

LPS-induced inflammation in chickens resulted in changes in the proportion and number of the two populations of splenic MRC1⁺cells. Chickens were administered PBS or LPS (1 mg/kg body weight) i.p. After 4 h, the spleens were collected, and the splenocytes were analysed to determine the number and proportional changes of the subtypes of MRC1⁺ cells by flow cytometry. A Representative dot plot with similar results showing MRC1⁺ cells from the spleen of chickens administered PBS or LPS. The solid line represents MRC1^loMHCII^hi cells, whereas the dashed line represents MRC1^hiMHCII^lo cells. The bar graph represents the B proportion and C absolute number of the two subsets in the PBS or LPS administration group (n = 10). Data are represented as the mean values ± SD. **P < 0.01, ***P < 0.001.