Splenic MRC1hiMHCIIlocells migrated towards CCL5 better than MRC1loMHCIIhicells.A-C Chicken splenocytes were sorted into MRC1loMHCIIhi and MRC1hiMHCIIlo cell populations using a cell sorter. The transcriptional levels of the chicken chemokine receptors A CCR5, B CCR2 and C CX3CR1 were analysed by RT-qPCR. Their expression was normalized to the β-actin mRNA level (n = 10). Data are represented as the mean values ± SD. ***P < 0.001. D, E For the in vitro migration assay, bead-sorted splenic MRC1+ cells (1 × 106 cells/mL) in 200 μL of media were placed in the insert of the transwell plate. The lower compartment was filled with media in the absence or presence of recombinant chicken CCL5 protein (50 ng/mL). After 1 h, D the percentage and E absolute number of migrated cells in the lower compartment were quantified by flow cytometry (n = 10). Data are represented as the mean values ± SD. **P < 0.01 ***P < 0.001.