Validation of RNA-Seq data by qPCR. ISGs were selected from ChIFN-α-stimulated DF1 cells (A); ChIFN-γ- stimulated DF1 cells (B); ChIFN-λ- stimulated DF1 cells (C); and ChIFN-λ- stimulated LMH cells (D). The data of relative mRNA expression level was derived from the ratio of the ChIFN-treatment group results to the control group results. qPCR and RNA-seq results were respectively displayed as 2−ΔΔCt value and the average log2 (fold change) values of DEG. Data from qPCR are representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. Error bars indicate SEM.