Skip to main content
Figure 8 | Veterinary Research

Figure 8

From: RNA-Seq analysis of European sea bass (Dicentrarchus labrax L.) infected with nodavirus reveals powerful modulation of the stress response

Figure 8

Modulation of the intracellular calcium content by NNV infection and the effect of cytoplasmic calcium availability on ROS production and NNV replication. A Intracellular calcium measurement with the fluorescent probe FLUO-4 AM in SSN-1 cells during infection with NNV. Values are the fold change of the fluorescence value obtained for the infected cells compared to the uninfected ones. B Calcium content measurement with FLUO-4 AM in brain cells isolated from in vivo NNV-infected sea bass and represented as the fold change value compared to the uninfected fish. C ROS production detection in head kidney leukocytes after in vitro pretreatment with the calcium chelators BAPTA-AM and EGTA and with or without NNV infection for 1 h. The data are represented as the fold change compared to untreated and uninfected cells. D Replication of NNV in SSN-1 cells after pretreatment with calcium channel blockers and chelators and infection with NNV for 48 h. Viral replication was detected by qPCR amplification of the gene encoding the NNV capsid.

Back to article page

Veterinary Research

ISSN: 1297-9716