Figure 6

Cortisol effects during NNV infection.A Kaplan–Meier survival curve representing the higher NNV susceptibility of sea bass carrying intraperitoneal slow-release cortisol implants compared to those inoculated with the vehicle alone. B NNV replication in the brain of sea bass carrying the cortisol implants or the vehicle alone (n = 5 individual samples). Viral replication was detected by qPCR amplification of the gene encoding the NNV capsid. C Expression of the ighm gene in NNV-infected or uninfected fish in the absence or presence of cortisol overload. The data are represented as fold change expression level in those animals carrying cortisol implants compared to those inoculated with the vehicle. D Detection of the IgM protein by ELISA in NNV-infected or uninfected fish carrying cortisol implants or the vehicle alone (n = 5 individual samples). The data are represented as fold change of protein abundance in those animals carrying cortisol implants compared to those inoculated with the vehicle. E Expression of the prl gene in NNV-infected or uninfected fish in the absence or presence of cortisol overload. The data are represented as fold change expression level in those animals carrying cortisol implants compared to those inoculated with the vehicle.