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Figure 6 | Veterinary Research

Figure 6

From: Porcine DNAJB6 promotes PCV2 replication via enhancing the formation of autophagy in host cells

Figure 6

The interaction between pDNAJB6 and Cap promotes the production of autophagosomes induced by PCV2 infection. A Wild-type PK-15 cells stably expressing EGFP-LC3 were transfected with pCI-neo, pCI-Cap or pCI-CapΔ162-234 (CapΔ162-234) plasmid for 24 h, respectively. The average number of green puncta was counted and calculated in 60 cells for each group. Results are shown as the mean ± SD (n = 3). **p < 0.01 versus pCI-Cap vector transfected cells. B, C PK-15 and PKpDNAJB6−/− cells stably expressing EGFP-LC3 were transfected with pCI-pDNAJB6, pCI-pDNAJB6ΔJ or blank vector (control) for 24 h, then cells were infected with PCV2 (B) or transfected with pCI-Cap plasmid (C) for 24 h. The average number of green puncta was counted and calculated in 60 cells in each group. Results are shown as the mean ± SD (n = 3). **p < 0.01 versus wild-type PK-15 cells. ##p < 0.01 versus PKpDNAJB6−/− cells that transfected with blank vector (PKpDNAJB6−/−/control).

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