The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Ognik, Katarzyna; Konieczka, Paweł; Mikulski, Dariusz; Jankowski, Jan

doi:10.1186/s13567-020-00776-y

Veterinary Research

Table 8 **Metabolic markers in the blood of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a high methionine (Met) level, challenged withClostridium perfringens—(Experiment 2).**

From: The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Item	CK U/L	ALT U/L	AST U/L	SOD U/gHb	GPx U/gHb	TP g/L	ALB g/L	UA mmol/L	UREA mmol/L	TC mmol/L	TG mmol/L	MDA µmol/L	GLU mmol/L
Arg₉₀*Infection (−)	0.249^d	13.13^a	239.0^c	807.2^c	14.65^d	44.59^a	20.51^b	0.412	1.319^ab	3.727^ab	0.621	1.718^b	6.95^c
Arg₉₀*Infection (+)	0.365^b	11.26^ab	275.4^ab	2234.1^a	20.80^c	36.05^b	18.97^bc	0.280	1.477^a	3.498^b	0.512	1.54^bc	9.43^b
Arg₁₀₀*Infection (−)	0.187^e	15.31^a	247.7^bc	1139.4^c	14.44^d	38.55^b	19.99^b	0.391	1.502^a	2.945^c	0.715	1.279^c	10.25^b
Arg₁₀₀*Infection (+)	0.168^e	6.64^c	303.7^a	2216.3^ab	47.94^b	38.1^b	23.60^a	0.347	1.146^ab	4.063^a	0.646	2.205^a	10.32^b
Arg₁₁₀*Infection (−)	0.236 ^cd	13.64^a	289.3^a	2126.6^ab	12.92^d	37.4^b	18.39^c	0.365	0.738^b	3.358^bc	0.396	1.830^b	12.02^a
Arg₁₁₀*Infection (+)	0.434^a	7.43^c	255.0^bc	1705.9^b	55.60^a	35.75^b	22.58^a	0.317	1.120^ab	4.243^a	0.336	2.119^a	12.10^a
SEM	0.014	0.678	4.438	93.23	2.576	0.676^b	0.305	0.011	0.067	0.079	0.024	0.074	0.290
Arg level, %
90	0.307^b	12.20	257.2^b	15207^c	17.72^b	40.32^a	19.74^b	0.346	1.398^a	3.613	0.566^b	1.629	8.19^c
100	0.177^c	10.98	275.7^a	1677.8^b	31.19^a	38.33^ab	21.80^a	0.369	1.324^a	3.504	0.681^a	1.742	10.28^b
110	0.335^a	10.53	272.2^ab	1916.3^a	34.26^a	36.58^b	20.48^ab	0.341	0.929^b	3.800	0.366^c	1.974	12.06^a
C. perfringens infection¹
–	0.224^b	14.03^a	258.7^b	1357.8^b	14.00^b	40.18^a	19.63^b	0.389^a	1.187	3.343^b	0.577^a	1.609^b	9.74^b
+	0.322^a	8.45^b	278.0^a	2052.1^a	41.45^a	36.64^b	21.72^a	0.315^b	1.248	3.935^a	0.498^b	1.955^a	10.62^a
P value
Arg	0.001	0.408	0.041	0.008	0.001	0.030	0.001	0.466	0.004	0.064	0.001	0.073	0.001
Infection	0.001	0.001	0.003	0.001	0.001	0.003	0.001	0.001	0.605	0.001	0.009	0.007	0.006
Arg × infection	0.001	0.034	0.001	0.001	0.001	0.009	0.001	0.124	0.040	0.001	0.755	0.003	0.002

Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg₉₀, Arg₁₀₀ and Arg₁₁₀, respectively) and a high level of Met (45% dietary Lys).
¹At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 10⁸ CFU/mL of bacteria. ^a,b,cMeans within a row with different superscripts differ significantly (P < 0.05).

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