Figure 2
From: Newcastle disease virus RNA-induced IL-1β expression via the NLRP3/caspase-1 inflammasome
NDV and IL-1β expression levels in the organs of SPF chickens and DF1 cells.A NDV M gene expression in the lungs, glandular stomach, and bursa of Fabricius of chickens. B IL-1β gene expression in the lungs, glandular stomach, and bursa of Fabricius of chickens. C NDV-NP protein expression in the lungs, glandular stomach, and bursa of Fabricius in chickens. D IL-1β protein expression in the lungs, glandular stomach, and bursa of Fabricius of chickens. The lungs, glandular stomach, and bursa of Fabricius were collected at 3 dpi, and levels of NDV and IL-1β were determined by qPCR, Western blot, or ELISA. E NDV M gene expression in DF1 cells. F IL-1β gene expression in DF1 cells. G NDV-NP protein expression in DF1 cells. H IL-1β protein expression in DF1 cells. DF1 cells and cell culture supernatant were collected at the indicated time points, and the levels of NDV and IL-1β were determined by qPCR, Western blot, or ELISA. *P < 0.05, **P < 0.01, and ***P < 0.001 between the experimental and control groups.