Figure 2

Validation of DEGs with qRT-PCR. IPEC-1 cells were challenged with ETEC, treated with EPS, or pretreated with EPS before ETEC challenge. Black bars—logFC values from RNA-seq; white bars—logFC values calculated from qRT-PCR. Pearson correlation coefficient (PCC) (r = 0.712 for the IPEC-1 cells exposed to ETEC^a; r = 0.728 for the IPEC-1 cells exposed to EPS^b; and r = 0.455 for the IPEC-1 cells exposed to EPS and ETEC^c; p < 0.01). Note that standard deviation (SD) is not shown here, as the expression levels (log² of the ΔΔCT values) of the DEGs in qRT-PCR was calculated based on average CT values of triplicates (the SD of CT ranged between 0.005 and 0.28).