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Table 2 Overview of the random amplification of polymorphic DNA (RAPD)-typing results for all samples harboring phenotypically different isolates from the same NAS species.

From: Fecal non-aureus Staphylococci are a potential cause of bovine intramammary infection

NAS species

Herd

Cow1

Quarter

Nisolates

Habitat

RAPD type2

S. chromogenes

Herd 1

Cow B

RF

2

QMC, TA

d, g

Cow F

RF

4

QMC

a, b, d3

S. devriesei

Herd 4

Cow A

LH

2

QMS

b, d

Cow J

LH

2

TA

a, b

S. equorum

Herd 2

Cow I

LF

2

TA

d, e

S. haemolyticus

Herd 1

Cow H

RF

2

QMH

c, d

Cow K

LH

2

TA

g

S. hominis

Herd 1

Cow B

LH

2

QMC

c, d

Cow G

LF

2

TA

e, e

Cow H

RF

4

TA

e, f

Cow H

RH

3

TA

f

Herd 2

Cow C

LF

3

TA

b, e

Cow E

–

2

RF

a, e

Cow F

–

3

RF

c, d

Cow G

–

2

RF

e, e

Cow I

RH

2

TA

g, m

Cow J

RH

3

TA

h, l

Herd 3

Cow G

LF

3

TA

a, g

Cow G

LH

2

TA

a

Herd 4

Cow C

LH

3

QMS

c

Cow K

LH

3

TA

b, c, i

Cow K

LF

2

TA

e, g

  1. 1Cows were assigned an arbitrary upper case letter, independently of the other dendrograms.
  2. 2RAPD types were assigned an arbitrary lower case letter based on the clustering.
  3. 3Cow F (RF quarter) had 2 clinical mastitis cases with an interval of 32 days. Staphylococcus chromogenes RAPD type a was isolated from the first clinical mastitis case and S. chromogenes RAPD type b and d were isolated from the second clinical mastitis case.
  4. HQuarter milk samples with a SCC ≤ 50 000 cells/mL milk (healthy).
  5. SQuarter milk samples with a SCC > 50 000 cells/mL milk (subclinical mastitis).
  6. CQuarter milk samples from a quarter with clinical signs of mastitis (clinical mastitis).
  7. CQuarter milk samples from a quarter with clinical signs of mastitis (clinical mastitis).