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Table 2 Genes investigated in this study and silencing effect

From: Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri

Gene

Average number of bacteria recovered

Mean Difference in number of bacteria recovered compared with control

Sig. of the effect of the silencing

Number of bacteria recovered as percentage of those recovered in the untreated control (%)

Scrambled RNA control

3.13E+05

  

100.0

Sec22B

1.05E+05

2.08E+05*

.004

33.4

ATPAse subunit F

1.05E+05

2.07E+05*

.001

33.7

VPS11

5.96E+04

2.53E+05*

.001

19.1

Rho GTPase

1.27E+05

1.86E+05*

.012

40.5

Ube2EL3

1.74E+05

1.39E+05a

.831a

55.7

sumo2

2.98E+04

2.83E+05*

.001

9.5

Nucleoside transporter 1

1.54E+04

2.97E+05*

.001

4.9

Integrin beta 1

1.57E+04

2.97E+05*

.001

5.0

Actin

1.98E+04

2.93E+05*

.001

6.3

Rac1

9.83E+03

3.03E+05*

.001

3.1

CDC42

2.78E+04

2.85E+05*

.001

8.9

arhgap18

2.61E+04

2.87E+05*

.001

8.3

lama2

9.43E+03

3.03E+05*

.001

3.0

β-Cadherin

3.04E+04

2.82E+05*

.001

9.7

Myotubularin protein 2

1.17E+04

3.01E+05*

.001

3.8

MApk14A

2.70E+04

2.86E+05*

.001

8.6

Caspase I precursor

1.85E+05

1.28E+05a

1.000a

59.1

Cyclin D1

1.03E+05

2.09E+05*

.005

33.1

ProtKinC

2.91E+04

2.84E+05*

.001

9.3

Rab1

3.54E+04

2.77E+05*

.001

11.3

Total

8.22E+04

   
  1. Silencing effect was determined by comparing the number of bacteria recovered at the end of the gentamicin assay. Significant difference between the results and the control are further indicated with an asterisk.
  2. aGenes (ubiquitin conjugating enzyme Ube2L3 and caspase I precursor) represent genes whose silencing did not significantly impact the gentamicin assay.
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Veterinary Research

ISSN: 1297-9716