Table 1 Primer sequences for the RT-qPCR confirmation of the siRNA silencing
From: Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
Ubiquitin* | GGAAAACCATCACCCTTGAG* |
ATAATGCCTCCACGAAGACG* | |
Elongation Factor 1 alpha* | GTCTACAAAATCGGCGGTAT* |
CTTGACGGACACGTTCTTGA* | |
Protein kinase C | TTGTTTGGCGGACTCCTGAA |
CCACGCCGAAAACAATCTCC | |
Rab-1A | AACCCCACCAACACCTCTAC |
GCACACACACACAACTTCTTTC | |
SEC22b-B | AGAGAGGAGAGGAGATAGGG |
TGGATAGAATGTGAGACCAGA | |
Vacuolar ATP synthase subunit A | CCGCTGAGGACTTCCGATAAAC |
ACAGCCTTCCATAGCATTGCAC | |
VPS-associated protein | ATCCACAACCTGACTGCCTACC |
CTCTCGCTGCTCTTGATGAAC | |
Rho1-GTPase | TAGCCCCAGTTTGCTCTTCG |
GCTGTCTCGTTCACTGGTCA | |
Ube213 | AACCCCACCTCCTTTCTCTC |
TTCACTAAGTCCCCTTGTTCC | |
Sumo 2 | TCCACCACAGAACCAATACC |
CAAAACAGGAAGGAAACAAAGG | |
Equilibrative nucleoside transporter 1 | ACGTCCGTGTTAAATGCTC |
ACAAAATCTCTCCTCCCCTC | |
Integrin β-1 precursor | ACCAATCCTACCCCCTATCC |
AGACCTCCCTCATTGTGTCG | |
Actin | CCAAGCAGGAATACGACGAG |
GCGTGGCAAAAAAAGTCCAG | |
Rac1 | ACTCACCCCCATTACCTACC |
CTGCTCCAATCTCTTTTGCC | |
SDC42 | AGAGGGGAAGGAATCAACAG |
ATGACAGGCAGGTAGGAGAG | |
Rho GTPase-activating prot. | CAGAAGAAAATGAGCAAAGACC |
CCTGAGAACTACTGTCCACC | |
Laminin 2 | GCGAATCAGAACCCTCAAC |
GCAGATACACATCCCTCCAAC | |
β-Cadherin | TCCCTCAGTTCCCTCAACTC |
TCTCAATCCTCTCCTCCTCC | |
Myotubularin-related protein 2 | TCAAGCGGAATACAAAAGACAG |
CCACTCTCTTCAACTCCTCATC | |
MaPK | TTCACTTCTCAAACCCCAAAC |
CGCTCAAAGGACAAACCAC | |
Caspase I precursor | GTGGAACAACAGGAAACAAAG |
TGATGGCAGTAGAACAGGG | |
Cyclin D1 | GCTGTCCTGTATGCTCTCTC |
CCCAAACCATTCCATTCTTCTC |