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Table 1 Specifications of the primary antibodies used for immunohistochemistry

From: Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica

Specificity

Type

Clone/product code

Dilution

Source

FDC

Mouse anti-human mAba

CNA.42/M7157

1:10

Dako

MHC-II

Mouse anti-human mAbb

HI2A/M0748

1:100

VMRD

CD83

Mouse anti-human mAba

HB15e/MCA1582GA

1:50

BioRad

CD1b

Mouse anti-bovine mAbc

CC20/MCA2058G

1:10

BioRad

S100

Rabbit pAba

-/Z0311

1:200

Dako

  1. mAb: monoclonal antibody, pAb: polyclonal antibody.
  2. aAntibody with cross-reactivity proved in sheep [13].
  3. bAntibody with cross-reactivity proved in sheep [66].
  4. cAntibody with cross-reactivity proved in sheep (manufacturer data sheet).
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Veterinary Research

ISSN: 1297-9716