Figure 6

Effect ofL. johnsoniiL531 pretreatment on CD4^+/−CCR6^+/−T cells and CCR6^+/−IFNγ^+/−T cells in peripheral blood. Peripheral blood samples were collected from the indicated piglets at 0, 24, and 192 h after S. Infantis challenge. Representative dot plots show the percentages of A CD4^+/− CCR6^+/− cells and E CCR6^+/− IFNγ^+/− cells among CD3⁺ T cells at 24 h after infection. Flow cytometry analysis of the percentages of B CD4⁻ CCR6⁺, C CD4⁺ CCR6⁺, D CD4⁺ CCR6⁻ cells and F CCR6⁻ IFNγ⁺, G CCR6⁺ IFNγ⁺, and H CCR6⁺ IFNγ⁻ cells within the peripheral CD3⁺ T-cell population in the indicated piglets. Data are presented as the mean ± SEM for each time point. *P < 0.05 (Tukey’s test).