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Figure 4 | Veterinary Research

Figure 4

From: Anti-inflammatory effects of Lactobacillus johnsonii L531 in a pig model of Salmonella Infantis infection involves modulation of CCR6+ T cell responses and ER stress

Figure 4

Lactobacillus johnsoniiL531 pretreatment attenuatesS. Infantis–induced ER stress in the small intestine. A Effect of L. johnsonii L531 on the structure of the rough ER in the small intestine after S. Infantis infection as observed using TEM. B, C Representative Western blot results for GRP78 and IRE1α in the ileum collected from piglets 10 days after S. Infantis challenge (upper panel). Results are presented as the ratio of the GRP78 or IRE1α band intensity to the intensity of the GAPDH band (lower panel). D, E Expression of ER marker gene mRNAs in both jejunal and ileal tissues collected from piglets 10 days after S. Infantis challenge analyzed using quantitative real-time PCR. Data are presented as the mean ± SEM for each tissue. *P < 0.05; **P < 0.01; ***P < 0.001 (Tukey’s test).

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