Isolation and characterization of a new population of nasal surface macrophages and their susceptibility to PRRSV-1 subtype 1 (LV) and subtype 3 (Lena)

Table 1 Antibodies used for immunofluorescence staining and flow cytometry

Primary antibodies	Clone	Isotype	Working dilution	Supplier
Anti-CD163 mAb	2A10/11	IgG1	1:200	Bio-rad
Anti-CD163 pAb	Polyclonal	IgG	1:200	R&D Systems
Anti-Sn mAb	41D3	IgG1	1:50	[27]
Anti-Sn mAb	26B2	IgG2b	1:30	[29]
Anti-MHCII mAb	MSA3	IgG2a	1:200	Kingfisher Biotech
Anti-CD14 mAb	MIL2	IgG2b	1:100	[28]
Anti-CD1c mAb	L161	IgG1	1:50	Biolegend
Anti-cytokeratin mAb	AE1/AE3	IgG1	1:50	Dako
Anti-vimentin mAb	V9	IgG1	1:50	Bio-rad
Anti-PRRSV nucleocapsid protein mAb	13E2	IgG2a	1:50	[32]
Anti-PCV2 Cap mAb	12E12	IgG2a	1:50	[30]
Anti-PRV gB mAb	1C11	IgG2b	1:100	[31]
Anti-PRV gD mAb	13D12	IgG1	1:50	[31]

ISSN: 1297-9716