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Figure 6 | Veterinary Research

Figure 6

From: Isolation and characterization of a new population of nasal surface macrophages and their susceptibility to PRRSV-1 subtype 1 (LV) and subtype 3 (Lena)

Figure 6

Characterization of the isolated primary nasal cells at different digestion times. A Double immunofluorescence staining of CD163 (red) in combination with Sn or vimentin or cytokeratin (green) in cells isolated after 24 h, 48 h, and 72 h digestion. CD163+ macrophages of various sizes are indicated with different arrows; yellow arrows (~25 µm), cyan arrows (~16 µm), and white arrows (~10 µm). Scale bar: 25 µm. B Percentage of positive cells from each time point are presented. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc test (*p < 0.05, **p < 0.01). All data are expressed as mean value of three experiments ± SD. C Flow cytometric analysis of the primary nasal cells collected after 72 h digestion.

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Veterinary Research

ISSN: 1297-9716