Figure 7

PCLS are susceptible to low pathogenic avian influenza virus strains. PCLS (500 μm) were generated from Hy-Line conventional (A) and PCLS (200–300 μm) were generated from PA12 SPF (B) chickens, infected with H7N1 (A) and H1N1 (B) LPAI virus strains, and stained for the viral proteins NP (A) and NS1 (B). Representative images showing uninfected PCLS (i) and virus-infected cells (green) within the PCLS at 24 (ii) and 48 (iii) hours post-infection. The nuclei are represented in blue. Images were captured by confocal microscopy at ×400 magnification. Scale bar = 10 µm (A) and 25 µm (B). n = 8 slices generated from 2 individual birds. Viral titre of supernatants harvested from H7N1-infected PCLS at 4, 24 and 48 h post-infection (C) and H1N1-infected PCLS at 8, 24 and 48 h post-infection (D). n = 8 slices generated from 2 individual birds, data are represented as the mean ± SD.