Figure 6

PCLS responses to LPS. PCLS (500 μm) prepared from from CSF1R-eGFP transgenic chickens and cultured in the presence (black) or absence (white) of LPS for 24 h on either day 1, 3, 5 or 7 post slice and supernatants harvested on day 2, 4, 6 and 8 post slice. IL-1β (A) and type I IFNs (B) production were measured in the supernatants using luciferase-based Mx- or NFκB-reporter bioassays. Results are expressed as fold change in luciferase activity relative to unstimulated PCLS. n = 3 slices generated from 2 individual birds. Nitrite produced by PCLS over 8 days of culture in response to LPS for 24 h was assessed in the supernatant by Griess assay (C). n = 6 slices generated from 4 individual birds. The concentration of IL-10 was assessed in PCLS supernatant by ELISA on day 2, 4 and 8 post slice (D). n = 6 slices generated from 4 individual birds. Data are represented as the mean ± SD.